By A H Rebar; et al
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Extra info for A Guide to Hematology in Dogs and Cats
Uses special acridine orange-coated tubes that include a cylindrical float with the same density as the buffy coat. When a tube is centrifuged, the float effectively reduces the diameter of the tube in the region of the buffy coat, causing it to spread along a greater length of the tube, enabling resolution of the various layers. ® The acridine orange stains nucleoproteins and other cellular components, which fluoresce when exposed to ultraviolet light. ® An automated reader (QBC® Vet Autoread, IDEXX Laboratories, Westbrook, Maine) scans the buffy coat layer and records the intensity of fluorescence produced by DNA and RNA/lipoproteins.
They can also occur in fragmentation hemolysis. A ghost RBC (arrow) is noted in the center of the field and suggests some degree of intravascular lysis (100x). Figure 4-26 Spherocytosis. If this were a feline blood smear, the RBC morphology would indicate regenerative anemia. The small RBC without central pallor would represent normal feline erythrocytes. However, this is canine blood and the small RBCs without central pallor are spherocytes. This dog has a marked spherocytosis due to immune-mediated hemolytic disease (100x).
Reticulocyte counts of greater than 80,000/µl indicate regeneration. Regenerative anemias are either the result of blood loss or hemolysis. Regenerative Anemia: Blood Loss / Hemorrhage History, clinical signs, and physical examination often point to the source of blood loss. In general, blood loss anemias are less responsive than hemolytic anemia. The main reason is that when blood loss occurs, iron is lost from the body and iron is needed for hemoglobin synthesis. If blood loss is severe or chronic, iron depletion and/or iron deficiency can occur, resulting in a nonregenerative anemia.
A Guide to Hematology in Dogs and Cats by A H Rebar; et al